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Life Science

PolyMax™ DNA Transfection Reagent

Catalog Number Unit Size Price
BC-PDT 1ml Please contact distributors
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상품 정보

상품 상세설명

Description

PolyMax™ is the transfection reagent which is PEI type (polyethyleneimine). This is powerful gene delivery compound that ensures higher efficiencies and reproducible transfection on eukaryotic cells such as HEK 293, COS-7, NIH-3T3, HeLa, CHO and specially mammalian cell lines.

Features

  • PEI type of transfection reagent
  • High Transfection Efficiency in Eukaryotic cells.

Storage

This product is stable for about one year from the shipment date.

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Protocols

General Procedures for Transfecting Mammalian Cells
Cell seeding
Cells should be plated 18 to 24 hours prior to transfection so that the monolayer cell density reaches the optimal 70-80 % confluency by the time of transfection.

PolyMax™-DNA Complex and Transfection Protocol
For different cell types, the optimal ratio of PolyMax™ (㎕) : DNA (㎍) is around 3 : 1. We recommend the PolyMax™ (㎕) : DNA (㎍) ratio of 3 : 1 as a starting point, as it usually gives satisfactory transfection efficiency with invisible cytotoxicity. To ensure the optimal size of PolyMax™/ DNA complex particles, we recommend using serum-free DMEM with High Glucose to dilute DNA and PolyMax™ Reagent.

The following protocol is given for transfection in 24-well plates. Refer to the table for transfection in other culture formats.

For each well, add 0.5 ㎖ of complete medium (with serum and antibiotics) 60 minutes before transfection.
For each well, dilute 0.5 ㎍ of DNA into 25 ㎕ of serum-free DMEM with High Glucose. Gently pipette up and down 3-5 times to mix.
In a new microtube, dilute 1.5 ㎕ of PolyMax™ reagent into 25 ㎕ of serum-free DMEM with High Glucose. Gently pipette up and down 3-5 times to mix.

Note: Never use medium containing serum and antibiotics, as it will disrupt the transfection complex.
Add ③ to ② right away, then pipette up and down 5-10 times to mix.
(Do not mix the solutions in the reverse order!)
Incubate at room temperature for 15 minutes to allow for the PolyMax™-DNA complexes to form. (Do not incubate for more than 20 minutes.)
Add 50 ㎕ of the PolyMax™-DNA complex drop-wise onto the medium in each well and homogenize the mixture by gently swirling the plate.
Remove PolyMax™/DNA complex-containing medium and replace with fresh complete medium 12-18 hours post transfection. (For sensitive cells, remove PolyMax™/DNA complex and replace with complete medium 5 hours after transfection to lower cytotoxicity.)
Measure transfection efficiency 24 - 48 hours post transfection.



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