Lactate dehydrogenase (LDH), which is released from cells damaged or killed by chemical or physical shock, is a stable enzyme present in the cytoplasm that does not pass through the cell membrane and is not released out of the cell. However, when the cell membrane is damaged or the cell dies, LDH is released outside the cell, and the amount of LDH in the medium is proportional to the number of dead or injured cells.

Quanti-LDH™ PLUS Cytotoxicity Assay Kit is a product that measures the cytotoxicity of LDH released from cells by the absorbance(490nm). It compensates for the disadvantages of Quanti-LDH products using chemical type electron transporters. By using chemical, stability at room temperature was improved and shelf life could be extended more than enzyme-based products. In addition, since cell viability can be measured, the time required for the experiment can be reduced.

 

Kit contents

Experimental Results

 

Ciatation

1) Ahn, H., Lee, G., Han, B.-C., Lee, S.-H., and Lee, G.-S. (2022). Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation. Antioxidants 11, 1923.

2) Min, J.-H., Cho, H.-J., and Yi, Y.-S. (2022). A novel mechanism of Korean Red Ginseng-mediated anti-inflammatory action via targeting caspase-11 non-canonical inflammasome in macrophages. Journal of Ginseng Research 46, 675-682. https://doi.org/10.1016/j.jgr.2021.12.009.

3) Lee, G., Ahn, H., Yun, J.-H., Park, J., Lee, E., Oh, S., and Lee, G.-S. (2022). JC2-11, a benzylideneacetophenone derivative, attenuates inflammasome activation. Scientific Reports 12, 22484. 10.1038/s41598-022-27129-3.

4) Wang Qian-wen, J.J.-s., Kim Jae Kyung (2022). Effects of heavy metals on the viability of A549 cells. Indian Journal of Biochemistry & Biophysics 59, 726-731.