Proteins transferred to Membrane for Western blot are subjected to a blocking process to prevent nonspecific antibody binding. Blocking buffer When making blocking buffer, animal proteins such as BSA are directly produced or purchased and used. However, in case of direct production, BSA denatures quickly, making it difficult to store for a long time even if it is always refrigerated. Even if a ready-to-use product is used, preservatives such as sodium azide are added to interfere with the antigen-antibody reaction, which prevents smooth Western blotting. Smart-Block™3% BSA Blocking Buffer uses specially designed preservatives to prevent denaturation due to protein contamination and is stable at room temperature. In addition, it does not show antibody-specific responses, so it can be used as an antibody dilution buffer as well as blocking.