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Life Science

MAX-Fluor™ Fluorometric Cell Viability Assay Kit

Catalog Number Unit Size
BCV-F500 500 tests
BCV-F1000 1,000 tests
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상품 정보

상품 상세설명

Description

MAX-Fluor™ Fluorometric Cell Viability Assay Kit is a fluorescence assay that allows cell viability testing to be performed at a high level of sensitivity comparable to isotopic labeling. The specially designed CytoFluor cannot penetrate the cell membrane, but CytoFluor-AM, which is an acetoxymethyl ester bond derivative, freely passes through the cell membrane. The fluorescent CytoFluor is then separated by intracellular esterase and stays inside the cell. It is measured by fluorescence microscope and FACS (Ex/Em = 490nm/515nm). At this time, the amount of fluorescence measured is determined by the membrane permeability of living cells and the activity of esterase, thus reflecting the activity of the cells. MAX-Fluor™ can be analyzed with much higher sensitivity (30~30,000 cells) than MTZ, MTS, WST and other tetrazolium salt methods. .

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Kit contents

Components

BCV-F500
(500 Tests)

BCV-F1000
(1000 Tests)

Storage

CytoFluor -AM in DMSO Solution

100 ㎕

100 ㎕ x 2 vials

-20 ℃


Experimental Note

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HEK-293 cells were grown to 24 hours in DMEM with 10% FBS and washed with PBS. EZ-Fluro™ in PBS treated to serially diluted cells in a black-walled microplate and incubated for 30 minutes at 37℃ under 5% CO₂ incubator.



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