MAX-Blue™ Cell Viability Assay Kit
- Life science
- MAX-Blue™ Cell Viability Assay Kit
The MAX-Blue™ Colorimetric / Fluorometric cell viability assay kit is a single-component ready-to-use solution. It is a cell viability test kit applicable to both absorbance and fluorescence measurement according to the usage type of the instrument. Fluorescence measurement shows a higher sensitivity than other tetrazolium salt measurement methods. The redox dye used in the product is reduced by metabolically active cells. Reduced dye can be measured at fluorescence (Ex/Em = 570/585nm) or absorbance (570nm). The detection sensitivity can be measured through a stable linear calibration value from about 102 to 105 cells. Since the dye does not forcibly interfere with the electron transfer flow of normal cells due to a very low reduction potential of the dye, it can be used in conjunction with other experiments.
Since the optimal linear calibration value is different for each cell, the initial setting time is required. Fluorescence measurements of MAX-Blue™solution for various cell concentrations. More than 100 cells start to show changes, and the optimal linear calibration values vary slightly from cell to cell.
1. Prepare about 100 μl of cell suspension in a 96-well plate at a density of (1 to 500) × 103 cells/well. Since the metabolic activity varies depending on the cell type, the number of cells required for the experiment also varies depending on the cell type. Set the appropriate cell concentration by preliminary testing.
2. Incubate in CO₂ incubator for 24 hours
3. Add the substance to be tested at various concentrations and incubate for 48 hours.
4. Add 10 μl of MAX-Blue™ solution and incubate for about 1 to 4 hours. Analyze fluorescence at ex = 530-560nm and Em = 590nm. (The appropriate incubation time differs from cell line to cell line, so measure the fluorescence or absorbance at an appropriate interval to determine the appropriate incubation time.) The absorbance can also be measured at 570nm/600nm or. However, it is recommended to use 540nm/600nm or 570nm/630nm. Both wavelengths cannot be changed (540 nm/630 nm cannot be used).