Biomax WestGlow™ ECL Chemiluminescent Substrate is a highly sensitivity reagent for the detection of proteins and antibodies that are blotted to the membrane. WestGlow™ Chemiluminescent Substrate enables detection of picogram range of protein and it can be used with both digital(LAS, Chemi-doc) and film-based imaging by reacting with horseradish peroxidase(HRP)-conjugated secondary antibody.

• Depending on the amount of protein, you can change the type of ECL. Use the same blotting condition when changing.
• When performing Western blotting, use a sufficient amount of blocking buffer, washing buffer, and chemiluminescent substrate. If you use too little amount of buffers, the background may go up.
• Do not store the membrane in a buffer containing EDTA or sodium azide. It can interfere to HRP.
• Do not touch the solution with your bare hands. It is recommended that you always use gloves and forceps when touching the membrane.
• Do not bend or hold the membrane hardly.
• Avoid light. In general, chemiluminescent substrates are very vulnerable to light.

For more information, please see the Western Blot Chemiluminescent Substrate selection guide

Ciatation

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3) Kim, H., Jang, J., Song, M.J., Kim, G., Park, C.H., Lee, D.H., Lee, S.H., and Chung, J.H. (2022). Attenuation of intrinsic ageing of the skin via elimination of senescent dermal fibroblasts with senolytic drugs. J Eur Acad Dermatol Venereol 36, 1125-1135. 10.1111/jdv.18051.
 

4) Ko, M.S., Yun, J.Y., Baek, I.J., Jang, J.E., Hwang, J.J., Lee, S.E., Heo, S.H., Bader, D.A., Lee, C.H., Han, J., et al. (2021). Mitophagy deficiency increases NLRP3 to induce brown fat dysfunction in mice. Autophagy 17, 1205-1221. 10.1080/15548627.2020.1753002.

5) Kang, W., Choi, D., Son, B., Park, S., and Park, T. (2022). Activation of OR10A3 by Suberic Acid Promotes Collagen Synthesis in UVB-Irradiated Dermal Fibroblasts via the cAMP-Akt Pathway. Cells 11, 3961.

6) Van Long, N., Chien, P.N., Tung, T.X., Van Anh, L.T., Giang, N.N., Nga, P.T., Linh, L.T.T., Nam, S.-Y., and Heo, C.-Y. (2023). Complementary combination of biomarkers for diagnosis of sarcopenia in C57BL/6J mice. Life Sciences 312, 121213. https://doi.org/10.1016/j.lfs.2022.121213.11, 3961.

7) Hao, S., Cho, B.O., Wang, F., Shin, J.Y., Shin, D.J., and Jang, S.I. (2022). Zingiber officinale attenuates neuroinflammation in LPS-stimulated mouse microglia by AKT/STAT3, MAPK, and NF-κB signaling. Food Science and Technology 42. 10.1590/fst.104221.