WestGlow™ PICO PLUS ECL Chemiluminescent Substrate
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Description
Biomax WestGlow™ PICO PLUS ECL Chemiluminescent Substrate is a highly sensitivity reagent for the detection of proteins and antibodies that are
blotted to the membrane. WestGlow™ PICO PLUS Chemiluminescent Substrate enables detection of low picogram to high femtogram range of protein and it can be used with both digital(LAS, Chemi-doc) and film-based imaging by reacting with horseradish peroxidase(HRP)-conjugated secondary antibody.
Providing the best quality and value in Chemiluminescent western blots
Sensitivity – Low picogram to High femtogram
Duration – 8 hours
Applicable – Digital and X-ray film imaging
Strength – Strong signal with no background
General note
- Depending on the amount of protein, you can change the type of ECL. Use the same blotting condition when changing.
- When performing Western blotting, use a sufficient amount of blocking buffer, washing buffer, and chemiluminescent substrate. If you use too little amount of buffers, the background may go up.
- Do not store the membrane in a buffer containing EDTA or sodium azide. It can interfere to HRP.
- Do not touch the solution with your bare hands. It is recommended that you always use gloves and forceps when touching the membrane.
- Do not bend or hold the membrane hardly.
- Avoid light. In general, chemiluminescent substrates are very vulnerable to light
For more information, please see the Western Blot Chemiluminescent Substrate selection guide.
Ciatation
2) Ko, S.B., Mihara, E., Park, Y., Roh, K., Kang, C., Takagi, J., Bang, I., and Choi, H.J. (2022). Functional role of the Frizzled linker domain in the Wnt signaling pathway. Commun Biol 5, 421. 10.1038/s42003-022-03370-4.