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Life Science

WestGlow™ PICO PLUS Chemiluminescent substrate

Catalog Number Unit Size
BWP0200 200ml (A+B)
BWP0400 400ml (A+B)
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상품 정보

상품 상세설명

Description

Biomax WestGlow™ PICO PLUS ECL Chemiluminescent Substrate is a highly sensitivity reagent for the detection of proteins and antibodies that are blotted to the membrane. WestGlow™ PICO PLUS Chemiluminescent Substrate enables detection of low picogram to high femtogram range of protein and it can be used with both digital(LAS, Chemi-doc) and film-based imaging by reacting with horseradish peroxidase(HRP)-conjugated secondary antibody.


Providing the best quality and value in Chemiluminescent western blots

Sensitivity – Low picogram to High femtogram
Duration – 8 hours
Applicable – Digital and X-ray film imaging
Strength – Strong signal with no background

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General note

  • Depending on the amount of protein, you can change the type of ECL. Use the same blotting condition when changing.
  • When performing Western blotting, use a sufficient amount of blocking buffer, washing buffer, and chemiluminescent substrate. If you use too little amount of buffers, the background may go up.
  • Do not store the membrane in a buffer containing EDTA or sodium azide. It can interfere to HRP.
  • Do not touch the solution with your bare hands. It is recommended that you always use gloves and forceps when touching the membrane.
  • Do not bend or hold the membrane hardly.
  • Avoid light. In general, chemiluminescent substrates are very vulnerable to light

For more information, please see the Western Blot Chemiluminescent Substrate selection guide.

Ciatation

1) Ahn, S., Yang, H., Son, S., Lee, H.S., Park, D., Yim, H., Choi, H.J., Swoboda, P., and Lee, J. (2022). The C. elegans regulatory factor X (RFX) DAF-19M module: A shift from general ciliogenesis to cell-specific ciliary and behavioral specialization. Cell Rep 39, 110661. 10.1016/j.celrep.2022.110661.


2) Ko, S.B., Mihara, E., Park, Y., Roh, K., Kang, C., Takagi, J., Bang, I., and Choi, H.J. (2022). Functional role of the Frizzled linker domain in the Wnt signaling pathway. Commun Biol 5, 421. 10.1038/s42003-022-03370-4.

3) Kim, H., Jang, J., Song, M.J., Kim, G., Park, C.H., Lee, D.H., Lee, S.H., and Chung, J.H. (2022). Attenuation of intrinsic ageing of the skin via elimination of senescent dermal fibroblasts with senolytic drugs. J Eur Acad Dermatol Venereol 36, 1125-1135. 10.1111/jdv.18051.
 

4) Ko, M.S., Yun, J.Y., Baek, I.J., Jang, J.E., Hwang, J.J., Lee, S.E., Heo, S.H., Bader, D.A., Lee, C.H., Han, J., et al. (2021). Mitophagy deficiency increases NLRP3 to induce brown fat dysfunction in mice. Autophagy 17, 1205-1221. 10.1080/15548627.2020.1753002.


5) Kang, W., Choi, D., Son, B., Park, S., and Park, T. (2022). Activation of OR10A3 by Suberic Acid Promotes Collagen Synthesis in UVB-Irradiated Dermal Fibroblasts via the cAMP-Akt Pathway. Cells 11, 3961.


6) Van Long, N., Chien, P.N., Tung, T.X., Van Anh, L.T., Giang, N.N., Nga, P.T., Linh, L.T.T., Nam, S.-Y., and Heo, C.-Y. (2023). Complementary combination of biomarkers for diagnosis of sarcopenia in C57BL/6J mice. Life Sciences 312, 121213. https://doi.org/10.1016/j.lfs.2022.121213.11, 3961.


7) Hao, S., Cho, B.O., Wang, F., Shin, J.Y., Shin, D.J., and Jang, S.I. (2022). Zingiber officinale attenuates neuroinflammation in LPS-stimulated mouse microglia by AKT/STAT3, MAPK, and NF-κB signaling. Food Science and Technology 42. 10.1590/fst.104221.

 


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