Biomax WestGlow™ FEMTO ECL Chemiluminescent Substrate is a highly sensitivity reagent for the detection of proteins and antibodies that are blotted to the membrane. WestGlow™ FEMTO Chemiluminescent Substrate enables detection of low picogram to high femtogram range of protein and it can be used with both digital(LAS, Chemi-doc) and film-based imaging by reacting with horseradish peroxidase(HRP)-conjugated secondary antibody.

Providing the best quality and value in Chemiluminescent western blots

Sensitivity – Mid femtogram to Low femtogram
Duration – 8 hours
Applicable – Digital and X-ray film imaging
Strength – Strong signal with no background

• Depending on the amount of protein, you can change the type of ECL. Use the same blotting condition when changing.
• When performing Western blotting, use a sufficient amount of blocking buffer, washing buffer, and chemiluminescent substrate. If you use too little amount of buffers, the background may go up.
• Do not store the membrane in a buffer containing EDTA or sodium azide. It can interfere to HRP.
• Do not touch the solution with your bare hands. It is recommended that you always use gloves and forceps when touching the membrane.
• Do not bend or hold the membrane hardly.
• Avoid light. In general, chemiluminescent substrates are very vulnerable to light.