PicoSens™ Acetylcholinesterase (AChE) Activity Assay Kit
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Description
PicoSens™ Acetylcholinesterase (AChE) Activity Assay Kit is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry. In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate(H2O2).
The intermediate reacts with a highly specific probe to generate color.

Kit contents
Components |
Size | Storage |
---|---|---|
AChE Assay Buffer | 25 ㎖ | -20℃ |
AChE Enzyme mix (Lyophilized) | 1 vial | |
AChE Substrate (Lyophilized) | 5 vials | |
AChE probe | 200 ㎕ | |
AChE Standard (50 mM) | 100 ㎕ |
Calculation
AChE activity = B/(ΔT x V) x D = nmol/min/ml =mU/ml