Description

Ascorbic Acid (Vitamin C) plays an important role in many biochemical processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. Therefore, it is important to be able to monitor ascorbic acid content in these different samples. The Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, enzyme mix oxidizes ascorbic acid to produce a by-product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/590 nm) methods.

 

Kit contents

Calculation

Concentration of ascorbic acid (C) = B/V x D (nmol/ ㎕ or nmol/ ㎕ or mM)