Description

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs (polyunsaturated fatty acids), are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such as malondialdehyde (MDA). The measurement of Thiobarbituric Acid Reactive Substances (TBARS) is a well-established method for screening and monitoring liquid peroxidation. MDA in the sample reacts with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct is easily quantified colorimetrically (532 nm). This analysis is based on the reaction of MDA at 25 ° C with TBA, a chromogenic reagent.

 

Kit contents

Calculation

 

Ciatation

1) Lee, D., Lee, K.-H., Kim, D.W., Yoon, S., and Cho, J.-Y. (2022). CXCL5 inhibits excessive oxidative stress by regulating white adipocyte differentiation. Redox Biology 54, 102359. https://doi.org/10.1016/j.redox.2022.102359.

2) Kim, J.A., Kim, M.J., Song, J.A., and Choi, C.Y. (2023). Effects of microfiber exposure on medaka (Oryzias latipes): Oxidative stress, cell damage, and mortality. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 265, 109535. https://doi.org/10.1016/j.cbpc.2022.109535.

3) Choi, J.H., Go, G.M., and Unno, T. (2022). Diatom frustule silica extracted from Melosira nummuloides ameliorates acetaminophen-induced acute liver injury in mice. Applied Biological Chemistry 65, 73. 10.1186/s13765-022-00741-7.