메인콘텐츠 바로가기
Life Science

Catalase assay kit

Catalog Number Unit Size
BO-CAT-400 400 assays
buy

상품 정보

상품 상세설명

Description

Catalase is an antioxidant enzyme present in most living plants and animals. This enzyme is known to be involved in the decomposition of H2O2 or reactive oxygen species (ROS), which are toxic by-products of aerobic metabolism. Catalase exhibits catalytic activity and peroxidatic activity. Through catalytic activity, catalase converts two molecules of H2O2 into one molecule of oxygen and two molecules of water. Through peroxidatic activity, catalase acts as an electron donor to oxidize low molecular weight alcohols. It is known to be involved in the H2O2 degradation reaction mainly in the liver, kidney, and erythrocytes in the human body.

OxiTec™ Catalase assay kit enables accurate catalase measurement with high sensitivity and a simple test method. It can measure catalase with the small amount of 50 mU/mL and use both fluorescence and absorbance measurement methods.


95fe7d10e10b0862ca81bd375ae351a8_1650336410_7284.gif
 

Kit contents

Components

Size

Storage

Oxi -Probe (MW=257)

200 ㎕

-20 ℃

Horseradish peroxidase (HRP, 20U) 1 vial
Hydrogen peroxide (H2O2 , 3%, 0.88M) 500 ㎕
5X Reaction buffer (pH 7.5, 0.5M) 20 ㎖
Catalase (100U) 1 vial

Calculation

06e6c5201337987011c68b6577250fad_1687147621_7045.jpg
 
 

Citations

1) Han, S.H., Ballinger, E., Choung, S.Y., and Kwon, J.Y. (2022). Anti-Photoaging Effect of Hydrolysates from Pacific Whiting Skin via MAPK/AP-1, NF-kappaB, TGF-beta/Smad, and Nrf-2/HO-1 Signaling Pathway in UVB-Induced Human Dermal Fibroblasts. Mar Drugs 20.


2) Hao, S., Cho, B.O., Wang, F., Shin, J.Y., Shin, D.J., and Jang, S.I. (2022). Zingiber officinale attenuates 6-hydroxydopamine induced oxidative stress and apoptosis through AKT, Nrf2, MAPK, NF-κB signaling pathway in PC12 cells. Food Science and Technology 42. 10.1590/fst.111221.


3) Kim, T.Y., Ku, H., and Lee, S.Y. (2020). Crop Enhancement of Cucumber Plants under Heat Stress by Shungite Carbon. Int J Mol Sci 21. 10.3390/ijms21144858.


4) Song, J.A., and Choi, C.Y. (2021). Temporal Changes in Physiological Responses of Bay Scallop: Performance of Antioxidant Mechanism in Argopecten irradians in Response to Sudden Changes in Habitat Salinity. Antioxidants 10, 1673. 10.3390/antiox10111673.


5) You, I.S., Sharma, S., Fadriquela, A., Bajgai, J., Thi, T.T., Rahman, M.H., Sung, J., Kwon, H.U., Lee, S.Y., Kim, C.S., and Lee, K.J. (2021). Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells. Molecules 26. 10.3390/molecules26216375.


6) Sharma, S., Lee, K.-J., Bajgai, J., Trinh, T.T., Antonio, J.M., Rahman, M.H., Vira, K., Sofian, A.-N., Cho, S.H., Kim, C.-S., and Kim, Y. (2022). Anti-Oxidative and Anti-Diabetic Effects of Electrolyzed Weakly Alkaline Reduced Water on Renal Proximal Tubular Epithelial Cells. Processes 10, 2025.



Keumkang Penterium IX Tower CORE-C 7F, 46, Galmaesunhwan-ro 166beon-gil, Guri-si, Gyeonggi-do, Republic of Korea
Tel : +82-3296-3158 I Fax : +82-973-2858 I E-mail : info@scgbiomax.com
CopyrightⓒBiomax All Rights Reserved.