Description
The Cholesterol/Cholesterol ester quantitation kit can be used to detect free cholesterol, cholesteryl esters, or both through quantitative analysis of absorbance (colorimetric) and fluorescence (fluorometric) measurements. Most of the cholesterol in blood exists in the form of cholesteryl esters which are hydrolyzed to free cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to produce H2O2 as a byproduct, which reacts with the cholesterol probe to produce color (maximum absorbance 570 nm) and fluorescence (Ex/Em = 535/590 nm). The assay detects free cholesterol in the absence of cholesterol esterase and total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase. The amount of cholesteryl ester is determined by taking the difference between the value of total cholesterol and the value of free cholesterol.
Kit contents
Components |
Size |
Storage |
|---|
| Lactate Assay Buffer |
25 ㎖ |
-20℃ |
| Lactate Enzyme mix (Lyophilized) |
1 vial |
| Lactate Probe |
200 ㎕ |
| Lactate Standard (100 mM) |
100 ㎕ |
Calculation
1)Koh, Y.I., Oh, K.S., Kim, J.A., Noh, B., Choi, H.J., Joo, S.Y., Rim, J.H., Kim, H.-Y., Kim, D.Y., Yu, S., et al. (2022). OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin. Autophagy 18, 2593-2614. 10.1080/15548627.2022.2040891.
2) Lee, D., Lee, K.-H., Kim, D.W., Yoon, S., and Cho, J.-Y. (2022). CXCL5 inhibits excessive oxidative stress by regulating white adipocyte differentiation. Redox Biology 54, 102359. https://doi.org/10.1016/j.redox.2022.102359.
3) Lee, J., Jeong, I., and Kim, O.-K. (2022). Liver-Derived Exosomes Induce Inflammation and Lipogenesis in Mice Fed High-Energy Diets. Nutrients 14, 5124. 90.
4) Park, S.-J., Kim, J.-L., Park, M.-R., Lee, J.W., Kim, O.-K., and Lee, J. (2022). Indian gooseberry and barley sprout mixture prevents obesity by regulating adipogenesis, lipogenesis, and lipolysis in C57BL/6J mice with high-fat diet-induced obesity. Journal of Functional Foods 90.
5) Kwon, M., Lim, S.-J., Joung, E.-J., Lee, B., Oh, C.-W., and Kim, H.-R. (2018). Meroterpenoid-rich fraction of an ethanolic extract from Sargassum serratifolium alleviates obesity and non-alcoholic fatty liver disease in high fat-fed C57BL/6J mice. Journal of Functional Foods 47, 288-298. https://doi.org/10.1016/j.jff.2018.05.063.
6) Jung, D.Y., Suh, N., and Jung, M.H. (2022). Tanshinone 1 prevents high fat diet-induced obesity through activation of brown adipocytes and induction of browning in white adipocytes. Life Sci 298, 120488. 10.1016/j.lfs.2022.120488.
