Description

Glucose (C6H12O6, FW: 180.16) is the primary biological fuel source used to generate the universal energy molecule, ATP. Glucose level is a key diagnostic parameter for many metabolic disorders and its measurement is very important in both research and drug discovery processes. The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, other body fluids, food, growth media, etc.). Glucose enzyme mix oxidizes glucose specifically, to generate a by-product which reacts with a dye to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color or fluorescence is directly proportional to the amount of glucose present. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.

Kit contents

Calculation

Glucose Concentration (C) = B/V x D (nmol/ µl or mM)

 

Ciatation

1) Lee, H.S., Jeon, E.Y., Nam, J.J., Park, J.H., Choi, I.C., Kim, S.H., Chung, J.J., Lee, K., Park, J.W., and Jung, Y. (2022). Development of a regenerative porous PLCL nerve guidance conduit with swellable hydrogel-based microgrooved surface pattern via 3D printing. Acta Biomater 141, 219-232. 10.1016/j.actbio.2022.01.042.

2) Lee, J., Jeong, I., and Kim, O.-K. (2022). Liver-Derived Exosomes Induce Inflammation and Lipogenesis in Mice Fed High-Energy Diets. Nutrients 14, 5124. 141, 219-232. 10.1016/j.actbio.2022.01.042.