Description

Glycerol (C3H8O3) is the main component of triglycerides, the most important storage form of fat. It is an important metabolite in energy metabolism involved in both oxidation and synthetic processes. Moreover, it is an important component of the production of foods and beverages, solvents, pharmaceutical and cosmetic products, etc. Under physiological conditions, triglycerides are hydrolyzed through lipolysis and release as glycerol and FFA (free fatty acid) in the blood. But unlike the FFA, glycerol cannot be reused by the adipose tissue. The Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm).

 

Kit contents

Calculation

Glycerol Concentration (C) = B/V x D (nmol/ ㎕ or mM)

Ciatation

1) Yeong Jun, J., Mi Gyeong, J., Jae-Won, K., Minyeong, K., Hee Chul, K., and Se Jae, K. (2022). The Anti-adipogenic and Lipolytic Effect of Jinkyool (Citrus sunki Hort. ex Tanaka) Leaf Extract in 3T3-L1 Cells. Journal of Life Science 32, 542-549.