Description

Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leukotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. The Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized (H2O2) with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (absorbance= 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

Kit contents

Calculation

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