Description

Regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)- cholesterol plays a central role in various disease developments. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events. PicoSensTM HDL and LDL/VLDL assay Kit provides a simple quantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL and VLDL (very low-density lipoprotein) in serum samples. In the assay, cholesterol oxidase specifically recognizes free cholesterol and produces products which react with probe to generate color (570 nm) and fluorescence (Ex/Em = 538/590 nm). Cholesterol esterase hydrolyzes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free cholesterol can be detected separately in the presence and absence of cholesterol esterase in the reactions.

 

Kit contents

Calculation

(C) = B/V x D (㎍/㎕)

Ciatation

1) Lee, J., Jeong, I., and Kim, O.-K. (2022). Liver-Derived Exosomes Induce Inflammation and Lipogenesis in Mice Fed High-Energy Diets. Nutrients 14, 5124.