There are various methods to quantify microorganisms in Biofilm, such as staining using crystal violet, microscopic observation, and measuring the amount of potassium extracted from microorganisms. Among them, Crystal Violet is a simple dyeing method for Dead cells, Live cells, Extracellular polysaccharides, etc. in Biofilm. It is widely used in Biofilm analysis using 96-well Microplate. It is highly reproducible, easy and fast to measure. Quanti-Micro^TM Biofilm Biomass Formation Assay Kit using crystal violet can quickly and simply measure the amount of biofilm formation and the biofilm formation inhibitory activity of the test substance.